Steric interactions between mobile ligands facilitate complete wrapping in passive endocytosis

Abstract

Receptor-mediated endocytosis is an ubiquitous process through which cells internalize biological or synthetic nanoscale objects, including viruses, unicellular parasites, and nanomedical vectors for drug or gene delivery. In passive endocytosis the cell plasma membrane wraps around the "invader" particle driven by ligand-receptor complexation. By means of theory and numerical simulations, here we demonstrate how particles decorated by freely diffusing and non-mutually-interacting (ideal) ligands are significantly more difficult to wrap than those where ligands are either immobile or interact sterically with each other. Our model rationalizes the relationship between uptake mechanism and structural details of the invader, such as ligand size, mobility and ligand/receptor affinity, providing a comprehensive picture of pathogen endocytosis and helping the rational design of efficient drug delivery vectors.

arXiv commentUpdated version of the manuscript. Accepted for publication in PRE

References in corpus (14)

• Wrapping of a spherical colloid by a fluid membrane
• The mechanism of phagocytosis: two stages of engulfment
• A general theory of DNA-mediated and other valence-limited interactions
• Volume and porosity thermal regulation in lipid mesophases by coupling mobile ligands to soft membranes
• Target shape dependence in a simple model of receptor-mediated endocytosis and phagocytosis
• Binding cooperativity of membrane adhesion receptors
• Effect of interactions on the cellular uptake of nanoparticles
• Binding constants of membrane-anchored receptors and ligands: a general theory corroborated by Monte Carlo simulations
• Theory and Simulation of DNA-Coated Colloids: a Guide for Rational Design
• An Effective Membrane Model of the Immunological Synapse
• Kinetic limitations of cooperativity based drug delivery systems
• Adhesion-induced phase separation of multiple species of membrane junctions
• Exploiting Receptors Competition to Enhance Nanoparticles Binding Selectivity
• A new configurational bias scheme for sampling supramolecular structures

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• Entropy-Driven Initiation and Cytoskeletal Viscoelasticity in Endocytosis: An Onsager Variational Framework