Phase-controlled dual-comb coherent anti-Stokes Raman spectroscopic imaging

Abstract

Coherent Raman microscopy provide label-free imaging by interrogating the intrinsic vibration of biomolecules. Nevertheless, trade-off between high chemical-specificity and high imaging-speed currently exists in transition from spectroscopy to spectroscopic imaging when capturing dynamics in complex living systems. Here, we present a novel concept in dual-comb scheme to substantially beat this trade-off and facilitate high-resolution broadband coherent anti-Stokes Raman spectroscopic imaging based on down-converted, automatically varying delay-time in spectral focusing excitation. A rapid measurement of vibrational microspectroscopy on sub-microsecond scale over a spectral span ~700 cm-1 with solid signal-to-noise ratio provides access to well-resolved molecular signatures within the fingerprint region. We demonstrate this high-performance spectroscopic imaging of spatially inhomogeneous distributions of chemical substances as well as the carotenoids accumulation in plant cells. This technique offers an unprecedented route for broadband CARS imaging with hundreds of kHz frame rate using available 1-GHz oscillators.

14 pages, 5 figures

References in corpus (7)

• Microresonator Soliton Dual-Comb Spectroscopy
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• Coherent Raman spectro-imaging with laser frequency combs
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Cited by in corpus (3)

• Ultra-high-speed coherent anti-Stokes Raman spectroscopy with a hybrid dual-comb source
• Broadband infrared spectroscopy of condensed phases with two intra-pulse difference-frequency-generation frequency combs
• Two-dimensional electronic spectroscopy of bacteriochlorophyll a with synchronized dual mode-locked lasers