paper

High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues

arXiv:1402.3211 · doi:10.1038/nphoton.2014.145

Abstract

We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique configuration of laser sources that probes the Raman spectrum over 3,000 cm and generates an especially strong response in the typically weak Raman "fingerprint" region through heterodyne amplification of the anti-Stokes photons with a large nonresonant background (NRB) while maintaining high spectral resolution of 13 cm. For histology and pathology, this system shows promise in highlighting major tissue components in a non-destructive, label-free manner. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumors and the surrounding healthy brain matter.

22 pages (11 main, 11 supplemental), 9 figures (4 main, 5 supplemental; N.B.: reduced resolution for smaller file size-- contact author for full resolution images). This paper was submitted to Nature Photonics on October 2, 2013 (minor corrections applied). This version concatenates the supplementary section to the end of the main text-- rather than a separate file-- No document changes otherwise

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