Superdiffusion dominates intracellular particle motion in the supercrowded space of pathogenic Acanthamoeba castellanii

Abstract

Acanthamoebae are free-living protists and human pathogens, whose cellular functions and pathogenicity strongly depend on the transport of intracellular vesicles and granules through the cytosol. Using high-speed live cell imaging in combination with single-particle tracking analysis, we show here that the motion of endogenous intracellular particles in the size range from a few hundred nanometers to several micrometers in Acanthamoeba castellanii is strongly superdiffusive and influenced by cell locomotion, cytoskeletal elements, and myosin II. We demonstrate that cell locomotion significantly contributes to intracellular particle motion, but is clearly not the only origin of superdiffusivity. By analyzing the contribution of microtubules, actin, and myosin II motors we show that myosin II is a major driving force of intracellular motion in A. castellanii. The cytoplasm of A. castellanii is supercrowded with intracellular vesicles and granules, such that significant intracellular motion can only be achieved by actively driven motion, while purely thermally driven diffusion is negligible.

23 pages, 9 figures

References in corpus (9)

• In vivo anomalous diffusion and weak ergodicity breaking of lipid granules
• Biological measurement beyond the quantum limit
• Single particle tracking in systems showing anomalous diffusion: the role of weak ergodicity breaking
• Viscoelastic subdiffusion: from anomalous to normal
• Quantitative analysis of single particle trajectories: mean maximal excursion method
• Localization Transition of the Three-Dimensional Lorentz Model and Continuum Percolation
• Geometry-induced superdiffusion in driven crowded systems
• Transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors
• Anomalous field-induced growth of fluctuations in dynamics of a biased intruder moving in a quiescent medium

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