Precise multi-emitter localization method for fast super-resolution imaging

Abstract

We present a method that can simultaneously locate positions of overlapped multi-emitters at the theoretical-limit precision. We derive a set of simple equations whose solution gives the maximum likelihood estimator of multi-emitter positions. We compare the performance of our simultaneous localization analysis with the conventional single-molecule analysis for simulated images and show that our method can improve the time-resolution of superresolution microscopy an order of magnitude. In particular, we derive the information-theoretical bound on time resolution of localization-based superresolution microscopy and demonstrate that the bound can be closely attained by our analysis.

5 pages, 2 figures

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