Nano-scale mechanical probing of supported lipid bilayers with atomic force microscopy

Abstract

We present theory and experiments for the force-distance curve $F(z_0)$ of an atomic force microscope (AFM) tip (radius $R$) indenting a supported fluid bilayer (thickness $2d$). For realistic conditions the force is dominated by the area compressibility modulus ${\kappa }_A$ of the bilayer, and, to an excellent approximation, given by $F=\pi {\kappa }_{A}R{z}_0^2/(2d-z_0{)}^2$. The experimental AFM force curves from coexisting liquid ordered and liquid disordered domains in 3-component lipid bilayers are well-described by our model, and provides ${\kappa }_A$ in agreement with literature values. The liquid ordered phase has a yield like response that we model by hydrogen bond breaking.

6 pages, 6 figures, accepted for publication in Physical Review E