Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns

Abstract

We reconstructed the 3D Fourier intensity distribution of mono-disperse prolate nano-particles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast X-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the Expansion-Maximization-Compression (EMC) framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.

4 pages, 4 figures

References in corpus (2)

• Femtosecond Diffractive Imaging with a Soft-X-ray Free-Electron Laser
• A reconstruction algorithm for single-particle diffraction imaging experiments

Cited by in corpus (18)

• Single-shot 3D structure determination of nanocrystals with femtosecond X-ray free electron laser pulses
• The Numerics of Phase Retrieval
• Phase Retrieval with Application to Optical Imaging
• Low-signal limit of X-ray single particle imaging
• Real-Space x-ray tomographic reconstruction of randomly oriented objects with sparse data frames
• Three-dimensional coherent Bragg imaging of rotating nanoparticles
• Orientation Determination in Single Particle X-ray Coherent Diffraction Imaging Experiments
• Progress in structure recovery from low dose exposures: Mixed molecular adsorption, exploitation of symmetry and reconstruction from the minimum signal level
• Three-Dimensional Coherent Diffractive Imaging of Isolated Faceted Nanostructures
• The Scatman: an approximate method for fast wide-angle scattering simulations
• Machine learning for ultrafast X-ray diffraction patterns on large-scale GPU clusters
• Assessing Uncertainties in X-ray Single-particle Three-dimensional reconstructions
• Atomic structure of a single large biomolecule from diffraction patterns of random orientations
• Reconstruction of 3D Image of Nanorice Particle from Randomly Oriented Single-Shot Experimental Diffraction Patterns Using Angular Correlation Method
• An encryption-decryption framework for validating single-particle imaging
• Solving Structure with Sparse, Randomly-Oriented X-ray Data
• Selection and orientation of different particles in single particle imaging
• Strategies for processing diffraction data from randomly oriented particles