output
20062025
most citedImprovement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients

457 citations

researchers with a paper here

Counting papers with at most 25 authors, so collaboration author lists do not stand in for the people here.

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12 papers · 1 filter

q-bio.GN2018★ 28 cited

Zinc oxide induces the stringent response and major reorientations in the central metabolism of Bacillus subtilis

Sylvie Luche, Elise Eymard-Vernain, Hélène Diemer +3

Microorganisms, such as bacteria, are one of the first targets of nanoparticles in the environment. In this study, we tested the effect of two nanoparticles, ZnO and TiO2, with the…

q-bio.GN2016★ 2 cited

A single step protein assay that is both detergent and reducer compatible: The cydex blue assay

Thierry Rabilloud

Determination of protein concentration in often an absolute pre-requisite in preparing samples for biochemical and proteomic analyses. However, current protein assay methods are no…

q-bio.GN2014★ 12 cited

Paleoproteomics explained to youngsters: how did the wedding of two-dimensional electrophoresis and protein sequencing spark proteomics on: Let there be light

Thierry Rabilloud

Taking the opportunity of the 20th anniversary of the word "proteomics", this young adult age is a good time to remember how proteomics came from enormous progress in protein separ…

q-bio.GN2014★ 21 cited

The proteomic to biology inference, a frequently overlooked concern in the interpretation of proteomic data: A plea for functional validation

Thierry Rabilloud, Pierre Lescuyer

Proteomics will celebrate its 20th year in 2014. In this relatively short period of time, it has invaded most areas of biology and its use will probably continue to spread in the f…

q-bio.GN2013★ 11 cited

How to use 2D gel electrophoresis in plant proteomics

Thierry Rabilloud

Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics tec…

q-bio.GN2013★ 4 cited

Multidimensional separation prior to mass spectrometry: Getting closer to the bottom of the iceberg

Manuel Mayr, Thierry Rabilloud

While prefractionation has previously been shown to improve results in MS analysis, a novel combination provides an additional dimension of separation: protein fractionation by SDS…

q-bio.GN2013★ 11 cited

When 2D is not enough, go for an extra dimension

Thierry Rabilloud

The use of an extra SDS separation in a different buffer system provide a technique for deconvoluting 2D gel spots made of several proteins (Colignon et al. Proteomics, 2013, 13, 2…

q-bio.GN2013★ 79 cited

Molecular responses of mouse macrophages to copper and copper oxide nanoparticles inferred from proteomic analyses

Sarah Triboulet, Catherine Aude-Garcia, Marie Carrière +10

The molecular responses of macrophages to copper-based nanoparticles have been investigated via a combination of proteomic and biochemical approaches, using the RAW264.7 cell line…

q-bio.GN2012★ 3 cited

Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myeloïd cell lines nuclear proteomes

Cécile Lelong, Mireille Chevallet, Hélène Diemer +3

One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear…

q-bio.GN2011★ 261 cited

Two-dimensional gel electrophoresis in proteomics: A tutorial

Thierry Rabilloud, Cécile Lelong

Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics,…

q-bio.GN2009★ 7 cited

Silver Staining of Proteins in 2DE Gels

Cécile Lelong, Mireille Chevallet, Sylvie Luche +1

Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and t…

q-bio.GN2006★ 457 cited

Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients

T. Rabilloud, C. Adessi, A. Giraudel +1

Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradient…