activity
20062018
most citedSilver staining of proteins in polyacrylamide gels

575 citations · 1.4k across the 20 of their papers we have counts for

collaborators

20 papers

q-bio.GN2018★ 28 cited

Zinc oxide induces the stringent response and major reorientations in the central metabolism of Bacillus subtilis

Sylvie Luche, Elise Eymard-Vernain, Hélène Diemer +3

Microorganisms, such as bacteria, are one of the first targets of nanoparticles in the environment. In this study, we tested the effect of two nanoparticles, ZnO and TiO2, with the…

q-bio.GN2012★ 3 cited

Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myeloïd cell lines nuclear proteomes

Cécile Lelong, Mireille Chevallet, Hélène Diemer +3

One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear…

q-bio.GN2012★ 76 cited

About the mechanism of interference of silver staining with peptide mass spectrometry

Sophie Richert, Sylvie Luche, Mireille Chevallet +3

The mechanism by which silver staining of proteins in polyacrylamide gels interferes with mass spectrometry of peptides produced by proteolysis has been investigated. It was demons…

q-bio.GN2011★ 7 cited

Effects of nanoparticles on murine macrophages

Mireille Chevallet, Catherine Aude-Garcia, Cécile Lelong +8

Metallic nanoparticles are more and more widely used in an increasing number of applications. Consequently, they are more and more present in the environment, and the risk that the…

q-bio.GN2011★ 17 cited

Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor man's fluorescent detection method

Catherine Aude-Garcia, Véronique Collin-Faure, Sylvie Luche +1

Fluorescent detection of proteins is a popular method of detection allying sensitivity, linearity and compatibility with mass spectrometry. Among the numerous methods described in…

q-bio.GN2010★ 16 cited

Two-dimensional gel electrophoresis in proteomics: past, present and future

Thierry Rabilloud, Mireille Chevallet, Sylvie Luche +1

Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of p…