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Biobeam - Rigorous wave-optical simulations of light-sheet microscopy
Martin Weigert, Eugene W. Myers, Moritz Kreysing
Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables to simul…
Isotropic reconstruction of 3D fluorescence microscopy images using convolutional neural networks
Martin Weigert, Loic Royer, Florian Jug +1
Fluorescence microscopy images usually show severe anisotropy in axial versus lateral resolution. This hampers downstream processing, i.e. the automatic extraction of quantitative…
Crowd Sourcing Image Segmentation with iaSTAPLE
Dmitrij Schlesinger, Florian Jug, Gene Myers +2
We propose a novel label fusion technique as well as a crowdsourcing protocol to efficiently obtain accurate epithelial cell segmentations from non-expert crowd workers. Our label…
Efficient Algorithms for Moral Lineage Tracing
Markus Rempfler, Jan-Hendrik Lange, Florian Jug +4
Lineage tracing, the joint segmentation and tracking of living cells as they move and divide in a sequence of light microscopy images, is a challenging task. Jug et al. have propos…