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Holographic characterization and tracking of colloidal dimers in the effective-sphere approximation
Lauren E. Altman, Rushna Quddus, Fook Chiong Cheong +1
An in-line hologram of a colloidal sphere can be analyzed with the Lorenz-Mie theory of light scattering to measure the sphere's three-dimensional position with nanometer-scale pre…
Dynamics of an acoustically trapped sphere in beating sound waves
Mohammed A. Abdelaziz, David G. Grier
A focused acoustic standing wave creates a Hookean potential well for a small sphere and can levitate it stably against gravity. Exposing the trapped sphere to a second transverse…
Holographic immunoassays
Kaitlynn Snyder, Rushna Quddus, Andrew D. Hollingsworth +2
The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an…
Interpreting Holographic Molecular Binding Assays with Effective Medium Theory
Lauren E. Altman, David G. Grier
Holographic molecular binding assays use holographic video microscopy to directly detect molecules binding to the surfaces of micrometer-scale colloidal beads by monitoring associa…
Quantitative differentiation of protein aggregates from other subvisible particles in viscous mixtures through holographic characterization
Annemarie Winters, Fook Chiong Cheong, Mary Ann Odete +5
We demonstrate the use of holographic video microscopy to detect individual subvisible particles dispersed in biopharmaceutical formulations and to differentiate them based on mate…
CATCH: Characterizing and Tracking Colloids Holographically using deep neural networks
Lauren E. Altman, David G. Grier
In-line holographic microscopy provides an unparalleled wealth of information about the properties of colloidal dispersions. Analyzing one colloidal particle's hologram with the Lo…