5 papers
Experimental demonstration of kinetic proofreading inherited in ligation-based information replication
Hiroyuki Aoyanagi, Yasuhiro Magi, Shoichi Toyabe
We experimentally demonstrate that information replication by templated ligation of DNA strands inherits a kinetic proofreading mechanism and achieves significant error suppression…
Acceleration of enzymatic reaction-diffusion kinetics by intermediate state
Akihiro Fukuda, Yohei Nakayama, Shoichi Toyabe
Biological molecular motors are high-performance nanomachines that convert chemical energy into mechanical motion via chemomechanical coupling. Their reaction cycles typically comp…
Asymmetric enzyme kinetics of F1-ATPase induced by rotation-assisted substrate binding
Yohei Nakayama, Shoichi Toyabe
We demonstrate asymmetric enzyme kinetics of a biomolecular motor F1-ATPase between synthesis and hydrolysis of adenosine triphosphate (ATP). Our experiments show that ATP hydrolys…
Efficiently driving F molecular motor in experiment by suppressing nonequilibrium variation
Takahide Mishima, Deepak Gupta, Yohei Nakayama +4
F-ATPase (F) is central to cellular energy transduction. Forcibly rotated by another motor F, F catalyzes ATP synthesis by converting mechanical work into…
Experimentally achieving minimal dissipation via thermodynamically optimal transport
Shingo Oikawa, Yohei Nakayama, Sosuke Ito +2
Optimal transport theory, originally developed in the 18th century for civil engineering, has since become a powerful optimization framework across disciplines, from generative AI…