10 citations · 10 across the 5 of their papers we have counts for
12 papers
PySTACHIO: Python Single-molecule TrAcking stoiCHiometry Intensity and simulatiOn, a flexible, extensible, beginner-friendly and optimized program for analysis of single-molecule microscopy
Jack W Shepherd, Ed J Higgins, Adam J M Wollman +1
As camera pixel arrays have grown larger and faster, and optical microscopy techniques ever more refined, there has been an explosion in the quantity of data acquired during routin…
Tetrameric UvrD helicase is located at the E. coli replisome due to frequent replication blocks
Adam J. M Wollman, Aisha H. Syeda, Andrew Leech +4
DNA replication in all organisms must overcome nucleoprotein blocks to complete genome duplication. Accessory replicative helicases in Escherichia coli, Rep and UvrD, help replicat…
Biophysical characterization of DNA origami nanostructures reveals inaccessibility to intercalation binding sites
Helen L . Miller, Sonia Contera, Adam J. M. Wollman +5
Intercalation of drug molecules into synthetic DNA nanostructures formed through self-assembled origami has been postulated as a valuable future method for targeted drug delivery.…
Single-molecule imaging of DNA gyrase activity in living Escherichia coli
Mathew Stracy, Adam J. M. Wollman, Elzbieta Kaja +9
Bacterial DNA gyrase introduces negative supercoils into chromosomal DNA and relaxes positive supercoils introduced by replication and transiently by transcription. Removal of thes…
Single molecule narrowfield microscopy of protein-DNA binding dynamics in glucose signal transduction of live yeast cells
Adam J. M. Wollman, Mark C. Leake
Single-molecule narrowfield microscopy is a versatile tool to investigate a diverse range of protein dynamics in live cells and has been extensively used in bacteria. Here, we desc…
Single-molecule observation of DNA replication repair pathways in E. coli
Adam J. M. Wollman, Aisha H. Syeda, Peter McGlynn +1
The method of action of many antibiotics is to interfere with DNA replication - quinolones trap DNA gyrase and topoisomerase proteins onto DNA while metronidazole causes single and…