204 citations · 498 across the 18 of their papers we have counts for
18 papers
Flight-Scope: microscopy with microfluidics in microgravity
Thomas Wareing, Alexander Stokes, Katrina Crompton +7
With the European Space Agency (ESA) and NASA working to return humans to the moon and onwards to Mars, it has never been more important to study the impact of altered gravity cond…
Membraneless organelles formed by liquid-liquid phase separation increase bacterial fitness
Xin Jin, Ji-Eun Lee, Charley Schaefer +10
Liquid-liquid phase separation is emerging as a crucial phenomenon in several fundamental cell processes. A range of eukaryotic systems exhibit liquid condensates. However, their f…
PySTACHIO: Python Single-molecule TrAcking stoiCHiometry Intensity and simulatiOn, a flexible, extensible, beginner-friendly and optimized program for analysis of single-molecule microscopy
Jack W Shepherd, Ed J Higgins, Adam J M Wollman +1
As camera pixel arrays have grown larger and faster, and optical microscopy techniques ever more refined, there has been an explosion in the quantity of data acquired during routin…
Tetrameric UvrD helicase is located at the E. coli replisome due to frequent replication blocks
Adam J. M Wollman, Aisha H. Syeda, Andrew Leech +4
DNA replication in all organisms must overcome nucleoprotein blocks to complete genome duplication. Accessory replicative helicases in Escherichia coli, Rep and UvrD, help replicat…
Biophysical characterization of DNA origami nanostructures reveals inaccessibility to intercalation binding sites
Helen L . Miller, Sonia Contera, Adam J. M. Wollman +5
Intercalation of drug molecules into synthetic DNA nanostructures formed through self-assembled origami has been postulated as a valuable future method for targeted drug delivery.…
Single-molecule imaging of DNA gyrase activity in living Escherichia coli
Mathew Stracy, Adam J. M. Wollman, Elzbieta Kaja +9
Bacterial DNA gyrase introduces negative supercoils into chromosomal DNA and relaxes positive supercoils introduced by replication and transiently by transcription. Removal of thes…
Transcription factor clusters regulate genes in eukaryotic cells
Adam J. M. Wollman, Sviatlana Shashkova, Erik G. Hedlund +3
Transcription is regulated through binding factors to gene promoters to activate or repress expression, however, the mechanisms by which factors find targets remain unclear. Using…
Single-molecule techniques in biophysics: a review of the progress in methods and applications
Helen Miller, Zhaokun Zhou, Jack Shepherd +2
Single-molecule biophysics has transformed our understanding of the fundamental molecular processes involved in living biological systems, but also of the fascinating physics of li…
An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond sampling time scales using bespoke Slimfield microscopy
Adam J. M. Wollman, Helen Miller, Simon Foster +1
Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image anal…
Single molecule narrowfield microscopy of protein-DNA binding dynamics in glucose signal transduction of live yeast cells
Adam J. M. Wollman, Mark C. Leake
Single-molecule narrowfield microscopy is a versatile tool to investigate a diverse range of protein dynamics in live cells and has been extensively used in bacteria. Here, we desc…
Single-molecule observation of DNA replication repair pathways in E. coli
Adam J. M. Wollman, Aisha H. Syeda, Peter McGlynn +1
The method of action of many antibiotics is to interfere with DNA replication - quinolones trap DNA gyrase and topoisomerase proteins onto DNA while metronidazole causes single and…
Designing a single-molecule biophysics tool for characterizing DNA damage for techniques that kill infectious pathogens through DNA damage effects
Helen Miller, Adam J. M. Wollman, Mark C. Leake
Antibiotics such as the quinolones and fluoroquinolones kill bacterial pathogens ultimately through DNA damage. They target the essential type IIA topoisomerases in bacteria by sta…
Automated image segmentation and division plane detection in single live Staphylococcus aureus cells
Adam J. M. Wollman, Helen Miller, Simon Foster +1
Staphylococcus aureus is a coccal bacterium, which divides by binary fission. After division the cells remain attached giving rise to small clusters, with a characteristic 'bunch o…
Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time
Adam J. M. Wollman, Mark C. Leake
We present a single-molecule tool called the CoPro (Concentration of Proteins) method that uses millisecond imaging with convolution analysis, automated image segmentation and supe…
Developing a new biophysical tool to combine magneto-optical tweezers with super-resolution fluorescence microscopy
Zhaokun Zhou, Helen Miller, Adam J. M. Wollman +1
We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse config…
From Animaculum to single-molecules: 300 years of the light microscope
Adam J. M. Wollman, Richard Nudd, Erik G. Hedlund +1
Although not laying claim to being the inventor of the light microscope, Antonj van Leeuwenhoek, (1632-1723) was arguably the first person to bring this new technological wonder of…
Probing DNA interactions with proteins using a single-molecule toolbox: inside the cell, in a test tube, and in a computer
Adam J. M. Wollman, Helen Miller, Zhaokun Zhou +1
DNA-interacting proteins have roles multiple processes, many operating as molecular machines which undergo dynamic metastable transitions to bring about their biological function.…
Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes
Helen Miller, Zhaokun Zhou, Adam J. M. Wollman +1
As proof-of-principle for generating superresolution structural information from DNA we applied a method of localization microscopy utilizing photoblinking comparing intercalating…