113 citations · 201 across the 17 of their papers we have counts for
4 papers · 1 filter
Single molecule narrowfield microscopy of protein-DNA binding dynamics in glucose signal transduction of live yeast cells
Adam J. M. Wollman, Mark C. Leake
Single-molecule narrowfield microscopy is a versatile tool to investigate a diverse range of protein dynamics in live cells and has been extensively used in bacteria. Here, we desc…
Using Fluorescence Recovery After Photobleaching (FRAP) to study dynamics of the Structural Maintenance of Chromosome (SMC) complex in vivo
Anjana Badrinarayanan, Mark C. Leake
The SMC complex, MukBEF, is important for chromosome organization and segregation in Escherichia coli. Fluorescently tagged MukBEF forms distinct spots (or 'foci') in the cell, whe…
Single-molecule observation of DNA replication repair pathways in E. coli
Adam J. M. Wollman, Aisha H. Syeda, Peter McGlynn +1
The method of action of many antibiotics is to interfere with DNA replication - quinolones trap DNA gyrase and topoisomerase proteins onto DNA while metronidazole causes single and…
Automated image segmentation and division plane detection in single live Staphylococcus aureus cells
Adam J. M. Wollman, Helen Miller, Simon Foster +1
Staphylococcus aureus is a coccal bacterium, which divides by binary fission. After division the cells remain attached giving rise to small clusters, with a characteristic 'bunch o…