Streptavidin-Biotin Binding is Intrinsically Non-Cooperative, but Experimental Artefacts Can Induce Apparent Positive Cooperativity

Malavika Kayyil Veedu, Eléonore Martenne Duplan, Saurabh Rai +2

The biotin-streptavidin interaction is a key component of nanobiotechnology, yet the nature of its cooperativity remains a debated question. Here, we introduce a label-free spectro…