3 papers
physics.bio-ph2026
Objective clustering protocol for single-molecule data: A lifetime vs. intensity study
Michael Lovemore, Joshua Botha, Bertus van Heerden +1
Single-molecule spectroscopy (SMS) is an exceptionally sensitive technique, but its inherently limited photon budget produces noisy data that can readily lead to subjective analyse…
physics.bio-ph2026
Size-Dependent Fluorescence Kinetics Reveal Contributions of Intrinsic Quenching and Singlet-Triplet Annihilation during LHCII Aggregation
Francois Conradie, Bertus van Heerden, Michal Gwizdala +1
Aggregation of the main antenna complex of higher plants, Light-Harvesting Complex II (LHCII), is widely used as an in vitro model for energy-dependent quenching (qE), yet fluoresc…
physics.bio-ph2024
Advanced analysis of single-molecule spectroscopic data
Joshua L. Botha, Bertus van Heerden, Tjaart P. J. Krüger
We present Full SMS, a multipurpose graphical user interface (GUI)-based software package for analysing single-molecule spectroscopy (SMS) data. SMS typically delivers multiparamet…