4 papers
Reverse engineering of mechano-kinetic parameters from stochastic force profiles in heterogeneous ensembles of molecular motors and tracks
Valentina Buonfiglio, Irene Pertici, Pasquale Bianco +2
Heterogeneity in contractile systems of molecular motors interacting with their tracks plays a key role in numerous cellular physiological and pathological processes. However, its…
Resolving the kinetics of an ensemble of muscle myosin motors via a temperature-dependent fitting procedure
Valentina Buonfiglio, Niccolò Zagli, Irene Pertici +3
A data fitting procedure is devised and thoroughly tested to provide self-consistent estimates of the relevant mechanokinetic parameters involved in a plausible scheme underpinning…
Force and kinetics of fast and slow muscle myosin determined with a synthetic sarcomere-like nanomachine
Valentina Buonfiglio, Irene Pertici, Matteo Marcello +7
Myosin II is the muscle molecular motor that works in two bipolar arrays in each thick filament of the striated (skeletal and cardiac) muscle, converting the chemical energy into s…
Kinetics of double stranded DNA overstretching revealed by 0.5-2 pN force steps
Pasquale Bianco, Lorenzo Bongini, Luca Melli +2
A detailed description of the conformational plasticity of double stranded DNA (ds) is a necessary framework for understanding protein-DNA interactions. Until now, however structur…