Sub-micromolar imaging of intrinsic chromophores by two-photon photothermal microscopy captures mitochondrial response to chemotherapy

Nathaniel Hai, Chinmayee Vallabh Prabhu Dessai, Dingcheng Sun +4

Intracellular chromophores (e.g., NADH and FAD) play a central role in regulation of cellular metabolism. Though autofluorescence has been extensively used for label-free mapping o…