Combining oligo pools and Golden Gate cloning to create protein variant libraries or guide RNA libraries for CRISPR applications

Alicia Maciá Valero, Rianne C. Prins, Thijs de Vroet +1

Oligo pools are array-synthesized, user-defined mixtures of single-stranded oligonucleotides that can be used as a source of synthetic DNA for library cloning. While currently offe…