5 papers
Chem-SIM: Super-resolution Chemical Imaging via Photothermal Modulation of Structured-Illumination Fluorescence
Dashan Dong, Danchen Jia, Xinyan Teng +6
Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop…
Sub-micromolar imaging of intrinsic chromophores by two-photon photothermal microscopy captures mitochondrial response to chemotherapy
Nathaniel Hai, Chinmayee Vallabh Prabhu Dessai, Dingcheng Sun +4
Intracellular chromophores (e.g., NADH and FAD) play a central role in regulation of cellular metabolism. Though autofluorescence has been extensively used for label-free mapping o…
Mid-wave infrared photothermal microscopy for molecular and metabolic imaging in deep tissues and spheroids
Mingsheng Li, Yuhao Yuan, Guangrui Ding +11
High-resolution chemical imaging within deep tissues and intact spheroids remains a grand challenge. Here, we introduce mid-wave infrared photothermal (MWIP) microscopy operating i…
Transfer-Function Approach to Substrate-Enhanced Diffraction Tomography
Tongyu Li, Yi Shen, Dashan Dong +4
Forward and backward scattering provide complementary volumetric and interfacial information, yet conventional three-dimensional (3D) imaging typically accesses only one. In this L…
Mid-Infrared Photothermal Relaxation Intensity Diffraction Tomography for Video-rate Volumetric Chemical Imaging
Danchen Jia, Dashan Dong, Tongyu Li +5
Three-dimensional molecular imaging of living cells is essential for unraveling cellular metabolism and response to therapies. However, existing volumetric methods, including fluor…