4 papers
Chem-SIM: Super-resolution Chemical Imaging via Photothermal Modulation of Structured-Illumination Fluorescence
Dashan Dong, Danchen Jia, Xinyan Teng +6
Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop…
FILM: Mapping organellar metabolism by mid-infrared photothermal modulated fluorescence
Jianpeng Ao, Jiaze Yin, Haonan Lin +10
Metabolism unfolds within specific organelles in eukaryotic cells. Lysosomes are highly metabolically active organelles, and their metabolic states dynamically influence signal tra…
Label-free Imaging of Single-Biomolecule Structure and Interaction by Stimulated Raman Photothermal Encoded Scattering
Pin-Tian Lyu, Yifan Zhu, Qing Xia +8
Current single molecule methods either rely on fluorescence or lack chemical information. Here we report stimulated Raman photothermal encoded scattering (SRPSCAT) microscopy for q…
Mid-wave infrared photothermal microscopy for molecular and metabolic imaging in deep tissues and spheroids
Mingsheng Li, Yuhao Yuan, Guangrui Ding +11
High-resolution chemical imaging within deep tissues and intact spheroids remains a grand challenge. Here, we introduce mid-wave infrared photothermal (MWIP) microscopy operating i…