3 papers
physics.optics2026
Mid-infrared photothermal imaging flow cytometry
Yusei Sugawara, Keiichiro Toda, Genki Ishigane +2
Imaging flow cytometry (IFC) enables high-throughput single-cell analysis but largely relies on fluorescence labeling to obtain molecular specificity. Label-free vibrational imagin…
physics.bio-ph2024
Label-free mid-infrared photothermal microscopy revisits intracellular thermal dynamics: what do fluorescent nanothermometers measure?
Keiichiro Toda, Masaharu Takarada, Genki Ishigane +4
Fluorescent nanothermometry has revealed pronounced intracellular temperature heterogeneity, establishing the field of single-cell thermal biology. However, these observations have…
physics.optics2023
Quantitative phase retrieval for Zernike phase-contrast microscopy
Rikimaru Kurata, Keiichiro Toda, Genki Ishigane +3
We present a single-image numerical phase retrieval method for Zernike phase-contrast microscopy (ZPM) that addresses halo and shade-off artifacts, as well as the weak phase condit…