14 citations · 66 across the 14 of their papers we have counts for
14 papers
Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells and in SMALP nanodiscs
Lukas Spantzel, Iván Pérez, Thomas Heitkamp +4
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor. The receptor is activated by a small peptide ligand neurotensin. NTSR1 can be expressed in HEK cells by st…
Fast ATP-dependent Subunit Rotation in Reconstituted FoF1-ATP Synthase Trapped in Solution
Thomas Heitkamp, Michael Börsch
FoF1-ATP synthases are ubiquitous membrane-bound, rotary motor enzymes that can catalyze ATP synthesis and hydrolysis. Their enzyme kinetics are controlled by internal subunit rota…
Observing monomer - dimer transitions of neurotensin receptors 1 in single SMALPs by homoFRET and in an ABELtrap
André Dathe, Thomas Heitkamp, Iván Pérez +5
G protein-coupled receptors (GPCRs) are a large superfamily of membrane proteins that are activated by extracellular small molecules or photons. Neurotensin receptor 1 (NTSR1) is a…
Ligand-induced oligomerization of the human GPCR neurotensin receptor 1 monitored in living HEK293T cells
Anika Westphal, Hendrik Sielaff, Stefanie Reuter +3
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor that can be expressed in HEK293T cells by stable transfection. Its ligand is a 13-amino-acid peptide that b…
Towards monitoring conformational changes of the GPCR neurotensin receptor 1 by single-molecule FRET
Thomas Heitkamp, Reinhard Grisshammer, Michael Börsch
Neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor that is important for signaling in the brain and the gut. Its agonist ligand neurotensin (NTS), a 13-amino-acid pepti…
Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy
Franziska Foertsch, Mykhailo Ilchenko, Thomas Heitkamp +6
Cytochrome C oxidase and FoF1-ATP synthase constitute complex IV and V, respectively, of the five membrane-bound enzymes in mitochondria comprising the respiratory chain. These enz…
Confining Brownian motion of single nanoparticles in an ABELtrap
Maria Dienerowitz, Thomas Heitkamp, Thomas Gottschall +2
Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and m…
Observing single FoF1-ATP synthase at work using an improved fluorescent protein mNeonGreen as FRET donor
Thomas Heitkamp, Gabriele Deckers-Hebestreit, Michael Börsch
Adenosine triphosphate (ATP) is the universal chemical energy currency for cellular activities provided mainly by the membrane enzyme FoF1-ATP synthase in bacteria, chloroplasts an…
Optimized green fluorescent protein fused to FoF1-ATP synthase for single-molecule FRET using a fast anti-Brownian electrokinetic trap
Maria Dienerowitz, Mykhailo Ilchenko, Bertram Su +5
Observation times of freely diffusing single molecules in solution are limited by the photophysics of the attached fluorescence markers and by a small observation volume in the fem…
Observing conformations of single FoF1-ATP synthases in a fast anti-Brownian electrokinetic trap
Bertram Su, Monika G. Dueser, Nawid Zarrabi +3
To monitor conformational changes of individual membrane transporters in liposomes in real time, we attach two fluorophores to selected domains of a protein. Sequential distance ch…
Regulatory conformational changes of the epsilon subunit in single FRET-labeled FoF1-ATP synthase
Thomas M. Duncan, Monika G. Dueser, Thomas Heitkamp +2
Subunit epsilon is an intrinsic regulator of the bacterial FoF1-ATP synthase, the ubiquitous membrane-embedded enzyme that utilizes a proton motive force in most organisms to synth…
Subunit rotation in single FRET-labeled F1-ATPase hold in solution by an anti-Brownian electrokinetic trap
Hendrik Sielaff, Thomas Heitkamp, Andrea Zappe +2
FoF1-ATP synthase catalyzes the synthesis of adenosine triphosphate (ATP). The F1 portion can be stripped from the membrane-embedded Fo portion of the enzyme. F1 acts as an ATP hyd…
Monitoring subunit rotation in single FRET-labeled FoF1-ATP synthase in an anti-Brownian electrokinetic trap
Thomas Heitkamp, Hendrik Sielaff, Anja Korn +3
FoF1-ATP synthase is the membrane protein catalyzing the synthesis of the 'biological energy currency' adenosine triphosphate (ATP). The enzyme uses internal subunit rotation for t…
Monitoring the conformational dynamics of a single potassium transporter by ALEX-FRET
N. Zarrabi, T. Heitkamp, J. C. Greie +1
Conformational changes of single proteins are monitored in real time by Forster-type resonance energy transfer, FRET. Two different fluorophores have to be attached to those protei…