Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly

Manos Mavrakis, Tsai Feng-Ching, Gijsje H. Koenderink

Septins are guanine nucleotide-binding proteins that are conserved from fungi to humans. Septins assemble into heterooligomeric complexes and higher-order structures with key roles…