22 citations · 83 across the 9 of their papers we have counts for
9 papers
Step size of the rotary proton motor in single FoF1-ATP synthase from a thermoalkaliphilic bacterium by DCO-ALEX FRET
Eva Hammann, Andrea Zappe, Stefanie Keis +5
Thermophilic enzymes can operate at higher temperatures but show reduced activities at room temperature. They are in general more stable during preparation and, accordingly, are co…
Monitoring transient elastic energy storage within the rotary motors of single FoF1-ATP synthase by DCO-ALEX FRET
Stefan Ernst, Monika G. Dueser, Nawid Zarrabi +1
The enzyme FoF1-ATP synthase provides the 'chemical energy currency' adenosine triphosphate (ATP) for living cells. Catalysis is driven by mechanochemical coupling of subunit rotat…
Subunit rotation in a single FoF1-ATP synthase in a living bacterium monitored by FRET
Karin Seyfert, Takuya Oosaka, Hideyuki Yaginuma +4
FoF1-ATP synthase is the ubiquitous membrane-bound enzyme in mitochondria, chloroplasts and bacteria which provides the 'chemical energy currency' adenosine triphosphate (ATP) for…
Drug transport mechanism of P-glycoprotein monitored by single molecule fluorescence resonance energy transfer
Stefan Ernst, Brandy Verhalen, Nawid Zarrabi +2
In this work we monitor the catalytic mechanism of P-glycoprotein (Pgp) using single-molecule fluorescence resonance energy transfer (FRET). Pgp, a member of the ATP binding casset…
Monitoring single membrane protein dynamics in a liposome manipulated in solution by the ABELtrap
Torsten Rendler, Marc Renz, Eva Hammann +3
FoF1-ATP synthase is the essential membrane enzyme maintaining the cellular level of adenosine triphosphate (ATP) and comprises two rotary motors. We measure subunit rotation in Fo…
Regulatory assembly of the vacuolar proton pump VOV1-ATPase in yeast cells by FLIM-FRET
Stefan Ernst, Claire Batisse, Nawid Zarrabi +2
We investigate the reversible disassembly of VOV1-ATPase in life yeast cells by time resolved confocal FRET imaging. VOV1-ATPase in the vacuolar membrane pumps protons from the cyt…