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physics.optics2025
Two-photon light-sheet live imaging at kilohertz frame rate using birefringence-based pulse splitting
Lei Zhu, Dale Gottlieb, Vincent Maioli +5
Multiphoton microscopy is widely used for live imaging. However, its acquisition speed remains limited by fluorophore emission rates and photodamage. To increase the pixel rate of…
physics.optics2025
Thermal Lensing Effects in Two-Photon Light-Sheet Microscopy
Antoine Hubert, Hugo Trentesaux, Thomas Pujol +2
In light-sheet fluorescence microscopy (LSFM), the axial resolution is governed by the illumination beam profile, motivating the development of advanced beam-shaping techniques to…