activity
20212025
most citedUnderstanding metric-related pitfalls in image analysis validation

204 citations · 672 across the 14 of their papers we have counts for

collaborators

14 papers

physics.bio-ph2025

GloBIAS: strengthening the foundations of BioImage Analysis

Agustin Andres Corbat, Christa G Walther, Laura Rodríguez de la Ballina +14

There is a global need for BioImage Analysis (BIA) as advances in life sciences increasingly rely on cutting-edge imaging systems that have dramatically expanded the complexity and…

q-bio.QM2025

Evaluating Integrative Strategies for Incorporating Phenotypic Features in Spatial Transcriptomics

Levin M Moser, Ahmad Kamal Hamid, Esteban Miglietta +2

Spatial transcriptomics (ST) technologies not only offer an unprecedented opportunity to interrogate intact biological samples in a spatially informed manner, but also set the stag…

cs.CV2025★ 1 cited

Foreground-aware Virtual Staining for Accurate 3D Cell Morphological Profiling

Alexandr A. Kalinin, Paula Llanos, Theresa Maria Sommer +11

Microscopy enables direct observation of cellular morphology in 3D, with transmitted-light methods offering low-cost, minimally invasive imaging and fluorescence microscopy providi…

q-bio.OT2024★ 8 cited

Creating and troubleshooting microscopy analysis workflows: common challenges and common solutions

Beth A Cimini

As microscopy diversifies and becomes ever-more complex, the problem of quantification of microscopy images has emerged as a major roadblock for many researchers. All researchers m…

q-bio.QM2024★ 43 cited

Cell Painting Gallery: an open resource for image-based profiling

Erin Weisbart, Ankur Kumar, John Arevalo +3

Image-based or morphological profiling is a rapidly expanding field wherein cells are "profiled" by extracting hundreds to thousands of unbiased, quantitative features from images…

q-bio.QM2023★ 16 cited

Defining the boundaries: challenges and advances in identifying cells in microscopy images

Nodar Gogoberidze, Beth A. Cimini

Segmentation, or the outlining of objects within images, is a critical step in the measurement and analysis of cells within microscopy images. While improvements continue to be mad…

q-bio.QM2023★ 47 cited

Reproducible image-based profiling with Pycytominer

Erik Serrano, Srinivas Niranj Chandrasekaran, Dave Bunten +25

Advances in high-throughput microscopy have enabled the rapid acquisition of large numbers of high-content microscopy images. Whether by deep learning or classical algorithms, imag…

eess.IV2023★ 151 cited

The Multi-modality Cell Segmentation Challenge: Towards Universal Solutions

Jun Ma, Ronald Xie, Shamini Ayyadhury +37

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or req…

cs.CV2023★ 2 cited

Pseudo-Labeling Enhanced by Privileged Information and Its Application to In Situ Sequencing Images

Marzieh Haghighi, Mario C. Cruz, Erin Weisbart +9

Various strategies for label-scarce object detection have been explored by the computer vision research community. These strategies mainly rely on assumptions that are specific to…

q-bio.QM2023★ 22 cited

CellProfiler plugins -- an easy image analysis platform integration for containers and Python tools

Erin Weisbart, Callum Tromans-Coia, Barbara Diaz-Rohrer +7

CellProfiler is a widely used software for creating reproducible, reusable image analysis workflows without needing to code. In addition to the >90 modules that make up the main Ce…

physics.optics2023★ 9 cited

Roadmap on Deep Learning for Microscopy

Giovanni Volpe, Carolina Wählby, Lei Tian +72

Through digital imaging, microscopy has evolved from primarily being a means for visual observation of life at the micro- and nano-scale, to a quantitative tool with ever-increasin…

cs.CV2023★ 204 cited

Understanding metric-related pitfalls in image analysis validation

Annika Reinke, Minu D. Tizabi, Michael Baumgartner +75

Validation metrics are key for the reliable tracking of scientific progress and for bridging the current chasm between artificial intelligence (AI) research and its translation int…

cs.DC2022★ 13 cited

Distributed-Something: scripts to leverage AWS storage and computing for distributed workflows at scale

Erin Weisbart, Beth A. Cimini

Distributed-Something coordinates the distribution of any Dockerized workflow using on-demand computational infrastructure from Amazon Web Services to enable at-scale workflows whe…

q-bio.QM2021★ 156 cited

CellProfiler Analyst 3.0: Accessible data exploration and machine learning for image analysis

David R. Stirling, Anne E. Carpenter, Beth A. Cimini

Image-based experiments can yield many thousands of individual measurements describing each object of interest, such as cells in microscopy screens. CellProfiler Analyst is a free,…