7 papers
Quantitative three-dimensional absorption imaging in standard brightfield microscopes
Yoonjae Chung, Sehyun Lee, Herve Hugonnet +5
Optical absorption is a primary, label-defining contrast across biology, pathology, and materials science, yet three-dimensional quantitative absorption imaging has remained largel…
Incoherent dielectric tensor tomography for quantitative 3D measurement of biaxial anisotropy
Juheon Lee, Yeon Wook Kim, Hwanseok Chang +4
Biaxial anisotropy, arising from distinct optical responses along three principal directions, underlies the complex structure of many crystalline, polymeric, and biological materia…
Pupil Phase Series: A Fast, Accurate, and Energy-Conserving Model for Forward and Inverse Light Scattering in Thick Biological Samples
Herve Hugonnet, Chulmin Oh, Juyeon Park +1
We present the pupil phase series (PPS), a fast and accurate forward scattering algorithm for simulating and inverting multiple light scattering in large biological samples. PPS ac…
Compression benchmarking of holotomography data using the OME-Zarr storage format
Dohyeon Lee, Juyeon Park, Juheon Lee +2
Holotomography (HT) is a label-free, three-dimensional imaging technique that captures refractive index distributions of biological samples at sub-micron resolution. As modern HT s…
Digital cytometry: extraction of forward and side scattering signals from holotomography
Jaepil Jo, Herve Hugonnet, Mahn Jae Lee +1
Flow cytometry is a cornerstone technique in medical and biological research, providing crucial information about cell size and granularity through forward scatter (FSC) and side s…
Rytov Approximation of Vectorial Waves by Modifying Scattering Matrixes: Precise Reconstruction of Dielectric Tensor Tomography
ChulMin Oh, Herve Hugonnet, Juheon Lee +1
Analyzing 3D anisotropic materials presents significant challenges, especially when assessing 3D orientations, material distributions, and anisotropies through scattered light, due…
High-precision and low-noise dielectric tensor tomography using a micro-electromechanical system mirror
Juheon Lee, Byung Gyu Chae, Hyuneui Kim +3
Dielectric tensor tomography is an imaging technique for mapping three-dimensional distributions of dielectric properties in transparent materials. This work introduces an enhanced…