8 papers
DART: A design-aware microfluidic chip paradigm for real-time live-cell image analysis
Johannes Seiffarth, Matthias Pesch, Lukas Scholtes +3
High-throughput microfluidic live-cell imaging generates rich single-cell data. Yet semi-automated procedures for locating regions of interest (RoIs), each containing one cell popu…
acia-workflows: Automated Single-cell Imaging Analysis for Scalable and Deep Learning-based Live-cell Imaging Analysis Workflows
Johannes Seiffarth, Keitaro Kasahara, Michelle Bund +7
Live-cell imaging (LCI) technology enables the detailed spatio-temporal characterization of living cells at the single-cell level, which is critical for advancing research in the l…
EAP4EMSIG -- Enhancing Event-Driven Microscopy for Microfluidic Single-Cell Analysis
Nils Friederich, Angelo Jovin Yamachui Sitcheu, Annika Nassal +12
Microfluidic Live-Cell Imaging (MLCI) yields data on microbial cell factories. However, continuous acquisition is challenging as high-throughput experiments often lack real-time in…
PyUAT: Open-source Python framework for efficient and scalable cell tracking
Johannes Seiffarth, Katharina Nöh
Tracking individual cells in live-cell imaging provides fundamental insights, inevitable for studying causes and consequences of phenotypic heterogeneity, responses to changing env…
How To Make Your Cell Tracker Say "I dunno!"
Richard D. Paul, Johannes Seiffarth, David Rügamer +2
Cell tracking is a key computational task in live-cell microscopy, but fully automated analysis of high-throughput imaging requires reliable and, thus, uncertainty-aware data analy…
EAP4EMSIG -- Experiment Automation Pipeline for Event-Driven Microscopy to Smart Microfluidic Single-Cells Analysis
Nils Friederich, Angelo Jovin Yamachui Sitcheu, Annika Nassal +12
Microfluidic Live-Cell Imaging (MLCI) generates high-quality data that allows biotechnologists to study cellular growth dynamics in detail. However, obtaining these continuous data…