22 citations · 137 across the 15 of their papers we have counts for
15 papers
Observing conformations of single FoF1-ATP synthases in a fast anti-Brownian electrokinetic trap
Bertram Su, Monika G. Dueser, Nawid Zarrabi +3
To monitor conformational changes of individual membrane transporters in liposomes in real time, we attach two fluorophores to selected domains of a protein. Sequential distance ch…
Subunit rotation in single FRET-labeled F1-ATPase hold in solution by an anti-Brownian electrokinetic trap
Hendrik Sielaff, Thomas Heitkamp, Andrea Zappe +2
FoF1-ATP synthase catalyzes the synthesis of adenosine triphosphate (ATP). The F1 portion can be stripped from the membrane-embedded Fo portion of the enzyme. F1 acts as an ATP hyd…
Monitoring subunit rotation in single FRET-labeled FoF1-ATP synthase in an anti-Brownian electrokinetic trap
Thomas Heitkamp, Hendrik Sielaff, Anja Korn +3
FoF1-ATP synthase is the membrane protein catalyzing the synthesis of the 'biological energy currency' adenosine triphosphate (ATP). The enzyme uses internal subunit rotation for t…
Manipulating freely diffusing single 20-nm particles in an Anti-Brownian Electrokinetic Trap (ABELtrap)
Nawid Zarrabi, Caterina Clausen, Monika G. Dueser +1
Conformational changes of individual fluorescently labeled proteins can be followed in solution using a confocal microscope. Two fluorophores attached to selected domains of the pr…
Monitoring transient elastic energy storage within the rotary motors of single FoF1-ATP synthase by DCO-ALEX FRET
Stefan Ernst, Monika G. Dueser, Nawid Zarrabi +1
The enzyme FoF1-ATP synthase provides the 'chemical energy currency' adenosine triphosphate (ATP) for living cells. Catalysis is driven by mechanochemical coupling of subunit rotat…
Drug transport mechanism of P-glycoprotein monitored by single molecule fluorescence resonance energy transfer
Stefan Ernst, Brandy Verhalen, Nawid Zarrabi +2
In this work we monitor the catalytic mechanism of P-glycoprotein (Pgp) using single-molecule fluorescence resonance energy transfer (FRET). Pgp, a member of the ATP binding casset…