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Background2Background: a self-supervised background removal framework for high dimensional microscopy
Guangrui Ding, Haonan Lin, Rong Tang +8
High-dimensional microscopy captures features across space, time, and spectrum, but complex backgrounds obscure signals. We present Background2Background (B2B), a self-supervised b…
Label-free Imaging of Single-Biomolecule Structure and Interaction by Stimulated Raman Photothermal Encoded Scattering
Pin-Tian Lyu, Yifan Zhu, Qing Xia +8
Current single molecule methods either rely on fluorescence or lack chemical information. Here we report stimulated Raman photothermal encoded scattering (SRPSCAT) microscopy for q…
Mid-wave infrared photothermal microscopy for molecular and metabolic imaging in deep tissues and spheroids
Mingsheng Li, Yuhao Yuan, Guangrui Ding +11
High-resolution chemical imaging within deep tissues and intact spheroids remains a grand challenge. Here, we introduce mid-wave infrared photothermal (MWIP) microscopy operating i…
Micromolar chemical imaging by high-energy low-photodamage Coherent Anti-stokes Raman Scattering (HELP-CARS)
Guangrui Ding, Dingcheng Sun, Yifan Zhu +5
Coherent anti-Stokes Raman scattering (CARS) microscopy offers label-free chemical imaging capabilities, but its performance is constrained by small Raman scattering cross-section,…