84 citations · 308 across the 6 of their papers we have counts for
6 papers
About the mechanism of interference of silver staining with peptide mass spectrometry
Sophie Richert, Sylvie Luche, Mireille Chevallet +3
The mechanism by which silver staining of proteins in polyacrylamide gels interferes with mass spectrometry of peptides produced by proteolysis has been investigated. It was demons…
Power and limitations of electrophoretic separations in proteomics strategies
Thierry Rabilloud, Ali R Vaezzadeh, Noelle Potier +3
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to…
Oxidative stress response: a proteomic view
Thierry Rabilloud, Mireille Chevallet, Sylvie Luche +1
The oxidative stress response is characterized by various effects on a range of biologic molecules. When examined at the protein level, both expression levels and protein modificat…
Improved mass spectrometry compatibility is afforded by ammoniacal silver staining
Mireille Chevallet, Hélène Diemer, Sylvie Luche +3
Sequence coverage in MS analysis of protein digestion-derived peptides is a key issue for detailed characterization of proteins or identification at low quantities. In gel-based pr…
Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view
Mireille Chevallet, Pierre Lescuyer, Hélène Diemer +3
The proper functioning of mitochondria requires that both the mitochondrial and the nuclear genome are functional. To investigate the importance of the mitochondrial genome, which…
About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis
Sylvie Luche, Hélène Diemer, Chistophe Tastet +4
The influence of thiol blocking on the resolution of basic proteins by two-dimensional electrophoresis was investigated. Cysteine blocking greatly increased resolution and decrease…