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Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor man's fluorescent detection method
Catherine Aude-Garcia, Véronique Collin-Faure, Sylvie Luche +1
Fluorescent detection of proteins is a popular method of detection allying sensitivity, linearity and compatibility with mass spectrometry. Among the numerous methods described in…
Power and limitations of electrophoretic separations in proteomics strategies
Thierry Rabilloud, Ali R Vaezzadeh, Noelle Potier +3
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to…
From secretome analysis to immunology: chitosan induces major alterations in the activation of dendritic cells via a TLR4-dependent mechanism
Christian Villiers, Mireille Chevallet, Hélène Diemer +5
Dendritic cells are known to be activated by a wide range of microbial products, leading to cytokine production and increased levels of membrane markers such as major histocompatib…
Membrane proteins and proteomics: Love is possible, but so difficult
Thierry Rabilloud
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully ba…
Silver Staining of Proteins in 2DE Gels
Cécile Lelong, Mireille Chevallet, Sylvie Luche +1
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and t…
Solubilization of Proteins in 2DE: An Outline
Thierry Rabilloud
Protein solubilization for two-dimensional electrophoresis (2DE) has to break molecular interactions to separate the biological contents of the material of interest into isolated a…