3 papers
q-bio.BM2025
The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states
Jamieson A L Howard, Benjamin Ambrose, Mahmoud A S Abdelhamid +10
DNA helicases undergo conformational changes; however, their structural dynamics are poorly understood. Here, we study single molecules of superfamily 1A DNA helicase Rep, which un…
q-bio.BM2021
RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity
Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd +2
The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA, RecBCD a helicase-nuclease that uses homologous recombination to resolve dou…
q-bio.CB2021
Investigating molecular crowding during cell division in budding yeast with FRET
Sarah Lecinski, Jack W Shepherd, Lewis Frame +3
Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with molecular changes in th…