85 citations · 235 across the 7 of their papers we have counts for
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From secretome analysis to immunology: chitosan induces major alterations in the activation of dendritic cells via a TLR4-dependent mechanism
Christian Villiers, Mireille Chevallet, Hélène Diemer +5
Dendritic cells are known to be activated by a wide range of microbial products, leading to cytokine production and increased levels of membrane markers such as major histocompatib…
Silver Staining of Proteins in 2DE Gels
Cécile Lelong, Mireille Chevallet, Sylvie Luche +1
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and t…
Solubilization of Proteins in 2DE: An Outline
Thierry Rabilloud
Protein solubilization for two-dimensional electrophoresis (2DE) has to break molecular interactions to separate the biological contents of the material of interest into isolated a…
Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis
Thierry Rabilloud
Because of the outstanding ability of two-dimensional electrophoresis to separate complex mixtures of intact proteins, it would be advantageous to apply it to all types of proteins…
Organelle proteomics
Pierre Lescuyer, Mireille Chevallet, Sylvie Luche +1
This unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with o…
Sweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry
Mireille Chevallet, Sylvie Luche, Hélène Diemer +3
Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness a…