5 papers
Using Azobenzene Photocontrol to Set Proteins in Motion
Olga Bozovic, Brankica Jankovic, Peter Hamm
Controlling the activity of proteins with azobenzene photoswitches is a potent tool for manipulating their biological function. With the help of light, one can change e.g. binding…
The Speed of Allosteric Signaling Within a Single-Domain Protein
Olga Bozovic, Jeannette Ruf, Claudio Zanobini +4
While much is known about different allosteric regulation mechanisms, the nature of the "allosteric signal", and the timescale on which it propagates, remains elusive. The PDZ3 dom…
Sequence of Events During Peptide Unbinding from RNase S: A Complete Experimental Description
Brankica Jankovic, Jeannette Ruf, Claudio Zanobini +3
The photo-triggered unbinding of the intrinsically disordered S-peptide from the RNase S complex is studied with the help of transient IR spectroscopy, covering a wide range of tim…
Intrinsic Dynamics of Protein-Peptide Unbinding
Brankica Jankovic, Olga Bozovic, Peter Hamm
The dynamics of peptide-protein binding and unbinding of a variant of the RNase S system has been investigated. To initiate the process, a photoswitchable azobenzene moiety has bee…
Real-time observation of ligand-induced allosteric transitions in a PDZ domain
Olga Bozovic, Claudio Zanobini, Adnan Gulzar +6
While allostery is of paramount importance for protein regulation, the underlying dynamical process of ligand (un)binding at one site, resulting time evolution of the protein struc…