550 citations · 1.1k across the 9 of their papers we have counts for
9 papers
FRET nanoscopy enables seamless imaging of molecular assemblies with sub-nanometer resolution
Jan-Hendrik Budde, Nicolaas van der Voort, Suren Felekyan +11
By circumventing the optical diffraction limit, super-resolved fluorescence microscopies enable the study of larger cellular structures and molecular assemblies. However, fluoresce…
Unraveling multi-state molecular dynamics in single-molecule FRET experiments -- Part II: Quantitative analysis of multi-state kinetic networks
Oleg Opanasyuk, Anders Barth, Thomas-Otavio Peulen +4
Single-molecule Förster Resonance Energy Transfer (smFRET) is ideally suited to resolve the dynamics of biomolecules. A significant challenge to date is capturing and quantifying t…
Unraveling multi-state molecular dynamics in single-molecule FRET experiments- Part I: Theory of FRET-Lines
Anders Barth, Oleg Opanasyuk, Thomas-Otavio Peulen +4
Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful approach to infor…
The FRET-based structural dynamics challenge -- community contributions to consistent and open science practices
Eitan Lerner, Benjamin Ambrose, Anders Barth +23
Single-molecule Förster resonance energy transfer (smFRET) has become a mainstream technique for probing biomolecular structural dynamics. The rapid and wide adoption of the techni…
Integrative dynamic structural biology unveils conformers essential for the oligomerization of a large GTPase
Thomas-Otavio Peulen, Carola S. Hengstenberg, Ralf Biehl +11
Guanylate binding proteins (GBPs) are soluble dynamin-like proteins with structured domains that undergo a conformational transition for GTP-controlled oligomerization to exert the…
Automated and optimally FRET-assisted structural modeling
Mykola Dimura, Thomas O. Peulen, Hugo Sanabria +4
FRET experiments can yield state-specific structural information on complex dynamic biomolecular assemblies. However, FRET experiments need to be combined with computer simulations…
Dynamic anticipation by Cdk2/Cyclin A-bound p27 mediates signal integration in cell cycle regulation
Maksym Tsytlonok, Hugo Sanabria, Yuefeng Wang +12
p27 (p27) is an intrinsically disordered protein (IDP) that folds upon binding to cyclin-dependent kinase (Cdk)cyclin complexes (e.g., Cdk2cyclin A), inhibiting thei…
Resolving dynamics and function of transient states in single enzyme molecules
Hugo Sanabria, Dmitro Rodnin, Katherina Hemmen +9
We used a hybrid fluorescence spectroscopic toolkit to monitor T4 Lysozyme (T4L) in action. By unraveling the kinetic and dynamic interplay of the conformational states, we sought…
Precision and accuracy of single-molecule FRET measurements - a worldwide benchmark study
Björn Hellenkamp, Sonja Schmid, Olga Doroshenko +50
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However…