82 citations · 208 across the 8 of their papers we have counts for
9 papers
Super-resolution Live-cell Fluorescence Lifetime Imaging
Raphaël Marchand, Henning Ortkrass, Daniel Aziz +7
Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution mi…
Fast Wide-field Light Sheet Electro-optic FLIM
V. Rose Knight, Nils Bode, Dara P. Dowlatshahi +5
We demonstrate volumetric fluorescence lifetime microscopy (FLIM) using the electro-optic FLIM technique. Images acquired in a selective plane illumination microscope are gated usi…
Wide-field fluorescence lifetime imaging of neuron spiking and sub-threshold activity in vivo
Adam J. Bowman, Cheng Huang, Mark J. Schnitzer +1
The development of voltage-sensitive fluorescent probes suggests fluorescence lifetime as a promising readout for electrical activity in biological systems. Existing approaches fai…
Nanosecond Photoemission near the Potential Barrier of a Schottky Emitter
Joshua L. Reynolds, Yonatan Israel, Adam J. Bowman +2
Nanosecond electron pulses are appealing for ultrafast imaging and electron gating applications, where tunable currents and narrow energy spreads are desirable. Here, we demonstrat…
Transmission Electron Microscopy at the Quantum Limit
Stewart A. Koppell, Yonatan Israel, Adam J. Bowman +2
A number of visions for a new generation of dose-efficient electron microscopes have been advanced. These proposals, while inspired by quantum principles, make little contact with…
Resonant Electro-optic Imaging for Microscopy at Nanosecond Resolution
Adam J. Bowman, Mark A. Kasevich
We demonstrate an electro-optic wide-field method to enable fluorescence lifetime microscopy (FLIM) with high throughput and single-molecule sensitivity. Resonantly driven Pockels…
RF pulse shaping for gated electron mirrors
Brannon B. Klopfer, Stewart A. Koppell, Adam J. Bowman +2
We present the design and prototype of a switchable electron mirror, along with a technique for driving it with a flat-top pulse. We employ a general technique for electronic pulse…
Design for a 10 KeV Multi-Pass Transmission Electron Microscope
Stewart A. Koppell, Marian Mankos, Adam J. Bowman +4
Multi-pass transmission electron microscopy (MPTEM) has been proposed as a way to reduce damage to radiation-sensitive materials. For the field of cryo-electron microscopy (cryo-EM…
Efficient wide-field FLIM
Adam J. Bowman, Brannon B. Klopfer, Thomas Juffmann +1
Nanosecond temporal resolution enables new methods for wide-field imaging like time-of-flight, gated detection, and fluorescence lifetime. The optical efficiency of existing approa…