8 papers
Femtosecond-to-millisecond holographic imaging of laser ablation dynamics
Shotaro Kawano, Keiichiro Toda, Miu Tamamitsu +3
Femtosecond laser ablation redistributes optically deposited energy across electronic, structural, mechanical, and thermal degrees of freedom over timescales from femtoseconds to m…
A unified framework for sensitivity comparison across stimulated Raman, stimulated Raman photothermal, and mid-infrared photothermal microscopy
Keiichiro Toda, Takuro Ideguchi
Label-free vibrational microscopy based on Raman scattering and mid-infrared (MIR) absorption has advanced biological imaging through the development of diverse high-sensitivity mo…
Mid-infrared photothermal imaging flow cytometry
Yusei Sugawara, Keiichiro Toda, Genki Ishigane +2
Imaging flow cytometry (IFC) enables high-throughput single-cell analysis but largely relies on fluorescence labeling to obtain molecular specificity. Label-free vibrational imagin…
Label-free mid-infrared photothermal microscopy revisits intracellular thermal dynamics: what do fluorescent nanothermometers measure?
Keiichiro Toda, Masaharu Takarada, Genki Ishigane +4
Fluorescent nanothermometry has revealed pronounced intracellular temperature heterogeneity, establishing the field of single-cell thermal biology. However, these observations have…
Video-rate volumetric chemical imaging via mid-infrared photothermal optical diffraction tomography
Masato Fukushima, Keiichiro Toda, Yusei Sugawara +2
Label-free vibrational microscopy provides chemically specific access to cellular structure, yet quantitative volumetric chemical dynamics in living cells remain largely inaccessib…
Bidirectional quantitative scattering microscopy
Kohki Horie, Keiichiro Toda, Takuma Nakamura +1
Quantitative phase microscopy (QPM) and interferometric scattering (iSCAT) microscopy are powerful label-free imaging techniques and are widely used for biomedical applications. Ea…