Nucleation and growth in one dimension, part II: Application to DNA replication kinetics

Abstract

Inspired by recent experiments on DNA replication, we apply a one-dimensional nucleation-and-growth model to DNA-replication kinetics, focusing on how to extract the time-dependent nucleation rate I(t) and growth speed v from data. We discuss generic experimental problems, namely spatial inhomogeneity, measurement noise, and finite-size effects. After evaluating how each of these affects the measurements of I(t) and v, we give guidelines for the design of experiments. These ideas are then discussed in the context of the DNA-replication experiments.

8 pages, 6 figures

References in corpus (3)

• Kinetic model of DNA replication in eukaryotic organisms
• Nucleation and growth in one dimension, part I: The generalized Kolmogorov-Johnson-Mehl-Avrami model
• Beyond the Kolmogorov Johnson Mehl Avrami kinetics: inclusion of the spatial correlation

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• Nucleation and growth in one dimension, part I: The generalized Kolmogorov-Johnson-Mehl-Avrami model
• How Xenopus laevis embryos replicate reliably: investigating the random-completion problem
• Optimal Placement of Origins for DNA Replication
• Beyond the constraints underlying Kolmogorov-Johnson-Mehl-Avrami theory related to the growth laws
• Quench dynamics of a dissipative Rydberg gas in the classical and quantum regime
• Genome replication in asynchronously growing microbial populations