Measurements of Protein-Protein Interactions by Size Exclusion Chromatography
arXiv:cond-mat/0306346 · doi:10.1016/S0006-3495(03)74684-0
Abstract
A method is presented for determining second virial coefficients B_2 of protein solutions from retention time measurements in size exclusion chromatography (SEC). We determine B_2 by analyzing the concentration dependance of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B_2 from positive to negative values in lysozyme and bovine serum albumin solutions. Our SEC results agree quantitatively with data obtained by light scattering.
18 pages including 1 table and 5 figures