Variable-Path-Length FTIR of E. coli in Aqueous Media

Abstract

Transmission infrared spectroscopy has been widely used for chemical analysis of biological samples in aqueous environments. However, its scope of applications has been limited by the path length, which is either too large or fixed, posing challenges for analyzing highly absorbing or heterogeneous samples. In this work, a mid-infrared (mid-IR) optical fiber probe was used for Fourier transform infrared (FTIR) micro-spectroscopy of aqueous Escherichia coli (E. coli) samples, providing continuous tuning of optical path length and sampling of near-surface and bulk regions. The mid-IR absorbance of the protein signal at 1548 cm-1 increased linearly with path length, consistent with the Beer-Lambert law. Path-length dependent spectra were used to calculate the spatially heterogeneous absorption coefficient of E. coli suspensions in aqueous media. The results demonstrate the ability of our fiber-based technique to resolve signals originating from different depths into the biological solution.