Tensorial tomographic Fourier Ptychography with applications to muscle tissue imaging
arXiv:2305.05085 · doi:10.1117/1.AP.6.2.026004
Abstract
We report Tensorial tomographic Fourier Ptychography (ToFu), a new non-scanning label-free tomographic microscopy method for simultaneous imaging of quantitative phase and anisotropic specimen information in 3D. Built upon Fourier Ptychography, a quantitative phase imaging technique, ToFu additionally highlights the vectorial nature of light. The imaging setup consists of a standard microscope equipped with an LED matrix, a polarization generator, and a polarization-sensitive camera. Permittivity tensors of anisotropic samples are computationally recovered from polarized intensity measurements across three dimensions. We demonstrate ToFu's efficiency through volumetric reconstructions of refractive index, birefringence, and orientation for various validation samples, as well as tissue samples from muscle fibers and diseased heart tissue. Our reconstructions of muscle fibers resolve their 3D fine-filament structure and yield consistent morphological measurements compared to gold-standard second harmonic generation scanning confocal microscope images found in the literature. Additionally, we demonstrate reconstructions of a heart tissue sample that carries important polarization information for detecting cardiac amyloidosis.
References in corpus (9)
- High-speed in vitro intensity diffraction tomography
- Diffraction tomography with a deep image prior
- High-throughput intensity diffraction tomography with a computational microscope
- Polarized light imaging of birefringence and diattenuation at highresolution and high sensitivity
- Deep learning-based holographic polarization microscopy
- Regularized Fourier Ptychography using an Online Plug-and-Play Algorithm
- Reference-free polarization-sensitive quantitative phase imaging using singe-point optical phase conjugation
- Phase and amplitude imaging with quantum correlations through Fourier Ptychography
- Attentional Ptycho-Tomography (APT) for three-dimensional nanoscale X-ray imaging with minimal data acquisition and computation time