Methods and measures for investigating microscale motility
arXiv:2303.00068 · doi:10.1093/icb/icad075
Abstract
Motility is an essential factor for an organism's survival and diversification. With the advent of novel single-cell technologies, analytical frameworks and theoretical methods, we can begin to probe the complex lives of microscopic motile organisms and answer the intertwining biological and physical questions of how these diverse lifeforms navigate their surroundings. Herein, we give an overview of different experimental, analytical, and mathematical methods used to study a suite of microscale motility mechanisms across different scales encompassing molecular-, individual- to population-level. We identify transferable techniques, pressing challenges, and future directions in the field. This review can serve as a starting point for researchers who are interested in exploring and quantifying the movements of organisms in the microscale world.
24 pages, 2 figures
References in corpus (13)
- The hydrodynamics of swimming microorganisms
- Hydrodynamic attraction of swimming microorganisms by surfaces
- Fluid dynamics and noise in bacterial cell-cell and cell-surface scattering
- Direct measurement of the flow field around swimming microorganisms
- Differential Dynamic Microscopy: Probing wave vector dependent dynamics with a microscope
- Ciliary contact interactions dominate surface scattering of swimming eukaryotes
- Bacterial hopping and trapping in porous media
- Differential Dynamic Microscopy: a High-Throughput Method for Characterizing the Motility of Microorganism
- Rhythmicity, Recurrence, and Recovery of Flagellar Beating
- The Bank Of Swimming Organisms at the Micron Scale (BOSO-Micro)
- How to Track Protists in Three Dimensions
- Shape mode analysis exposes movement patterns in biology: flagella and flatworms as case studies
- Rebound and scattering of motile Chlamydomonas algae in confined chambers