Light-field microscopy with correlated beams for extended volumetric imaging at the diffraction limit

Abstract

Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture of information on light spatial distribution and propagation direction. However, state-of-the-art light-field microscopes suffer from a detrimental loss of spatial resolution compared to standard microscopes. We propose and experimentally demonstrate a light-field microscopy architecture based on light intensity correlation, in which resolution is limited only by diffraction. We demonstrate the effectiveness of our technique in refocusing three-dimensional test targets and biological samples out of the focused plane. We improve the depth of field by a factor 6 with respect to conventional microscopy, at the same resolution, and obtain, from one acquired correlation image, about $130,000$ images, all seen from different perspectives; such multi-perspective images are employed to reconstruct over $40$ planes within a $1{\mathrm{mm}}^3$ sample with a diffraction-limited resolution voxel of $20\times 20\times 30\mu {\mathrm{m}}^3$.

14 pages, 10 figures

References in corpus (5)

• Correlated imaging, quantum and classical
• Realisation of the first sub shot noise wide field microscope
• Beating Abbe diffraction limit in confocal microscopy via non-classical photon statistics
• Signal-to-noise properties of correlation plenoptic imaging with chaotic light
• Exploring plenoptic properties of correlation imaging with chaotic light

Cited by in corpus (5)

• Correlated-photon imaging at 10 volumetric images per second
• Light Field Ghost Imaging
• Quantum correlation light-field microscope with extreme depth of field
• Light-field imaging from position-momentum correlations
• Robustness of chaotic-light correlation imaging against turbulence