One-step, Wash-free, Nanoparticle Clustering-based Magnetic Particle Spectroscopy (MPS) Bioassay Method for Detection of SARS-CoV-2 Spike and Nucleocapsid Proteins in Liquid Phase

Abstract

With the ongoing global pandemic of coronavirus disease 2019 (COVID-19), there is an increasing quest for more accessible, easy-to-use, rapid, inexpensive, and high accuracy diagnostic tools. Traditional disease diagnostic methods such as qRT-PCR (quantitative reverse transcription-PCR) and ELISA (enzyme-linked immunosorbent assay) require multiple steps, trained technicians, and long turnaround time that may worsen the disease surveillance and pandemic control. In sight of this situation, a rapid, one-step, easy-to-use, and high accuracy diagnostic platform will be valuable for future epidemic control especially for regions with scarce medical resources. Herein, we report a magnetic particle spectroscopy (MPS) platform for detection of SARS-CoV-2 biomarkers: spike and nucleocapsid proteins.

arXiv comment35 pages, 14 figures

References in corpus (4)

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• Simulations of magnetic nanoparticle Brownian motion
• Magnetic Nanoparticle Relaxation Dynamics-based Magnetic Particle Spectroscopy (MPS) for Rapid and Wash-free Molecular Sensing
• Portable Magnetic Particle Spectrometer (MPS) for Future Rapid and Wash-free Bioassays