Modulation of the cardiomyocyte contraction inside a hydrostatic pressure bioreactor: $\text{in vitro}$ verification of the Frank-Starling law

Abstract

We have studied beating mouse cardiac syncytia $\text{in vitro}$ in order to assess the inotropic, ergotropic, and chronotropic effects of both increasing and decreasing hydrostatic pressures. In particular, we have performed an image processing analysis to evaluate the kinematics and the dynamics of those pressure-loaded beating syncytia starting from the video registration of their contraction movement. By this analysis, we have verified the Frank-Starling law of the heart in $\text{in vitro}$ beating cardiac syncytia and we have obtained their geometrical-functional classification.