Deep penetration fluorescence imaging through dense yeast cells suspensions using Airy beams

Abstract

We propose a new method to image fluorescent objects through turbid media base on Airy beam scanning. This is achieved by using the non-diffractive nature of Airy beams, namely their ability to maintain their shape while penetrating through a highly scattering medium. We show, that our technique can image fluorescent objects immersed in turbid media with higher resolution and signal to noise than confocal imaging. As proof-of-principle, we demonstrate imaging of 1$\mu$m sized fluorescent beads through a dense suspension of yeast cells with an attenuation coefficient of 51cm${}^{-1}$ at a depth of 90$\mu$m. Finally, we demonstrate that our technique can also provide the depth of the imaged object without any additional sectioning.

5 pages, 5 figures

References in corpus (1)

• Non-invasive real-time imaging through scattering layers and around corners via speckle correlations