End-to-end distance and contour length distribution functions of DNA helices

Abstract

We present a computational method to evaluate the end-to-end and the contour length distribution functions of short DNA molecules described by a mesoscopic Hamiltonian. The method generates a large statistical ensemble of possible configurations for each dimer in the sequence, selects the global equilibrium twist conformation for the molecule and determines the average base pair distances along the molecule backbone. Integrating over the base pair radial and angular fluctuations, we derive the room temperature distribution functions as a function of the sequence length. The obtained values for the most probable end-to-end distance and contour length distance, providing a measure of the global molecule size, are used to examine the DNA flexibility at short length scales. It is found that, also in molecules with less than $\sim 60$ base pairs, coiled configurations maintain a large statistical weight and, consistently, the persistence lengths may be much smaller than in kilo-base DNA.

10 pages, 7 figures

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• Strongly bent double-stranded DNA: reconciling theory and experiment
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• Distributions of Bubble Lifetimes and Bubble Lengths in DNA
• Bubble lifetimes in DNA gene promoters and their mutations affecting transcription
• Base pair fluctuations in helical models for nucleic acids
• Short DNA persistence length in a mesoscopic helical model
• Sharp DNA denaturation in a helicoidal mesoscopic model
• First-passage probability: a test for DNA Hamiltonian parameters
• Stretching DNA in hard-wall potential channels
• Mesoscopic helical models for DNA
• Twist-stretch relations in nucleic acids
• Mesoscopic model for nano-channel confined DNA
• Statistical method for A-RNA and B-DNA