Brillouin microscopy, what is it really measuring?
arXiv:1711.03312 · doi:10.1038/s41592-018-0076-1
Abstract
Brillouin microscopy measures compressibility, but is being increasingly used to assess stiffness of cells and tissues. Using hydrogels with tunable properties, we demonstrate that Brillouin microscopy is insensitive to stiffness of hydrated materials, but depends strongly on water content, consistent with a theoretical model of biphasic compressibility. Empirical correlations between Brillouin measurements and stiffness arise due to their mutual dependence on water content, but correlations vanish once hydration is controlled.
arXiv commentUpdated version with expanded discussion, additional data and a mathematical model to relate Brillouin measurements to water content
Cited by in corpus (6)
- Brillouin Light Scattering: Applications in Biomedical Sciences
- Brillouin-derived viscoelastic parameters of hydrogel tissue models
- Multivariate analysis of Brillouin imaging data by supervised and unsupervised learning
- Brillouin microscopy - a revolutionary tool for mechanobiology?
- Precision and informational limits in inelastic optical spectroscopy
- Background-free fibre optic Brillouin probe for remote mapping of micromechanics